Flyby-Denial Presents: Protein Comparison Pipeline
A bioinformatics pipeline built in Google Colab for analyzing and comparing protein sequences. Designed to be straightforward to use and modify, making it accessible for students and researchers exploring protein analysis for the first time.
The pipeline accepts protein sequences either as uploaded FASTA files or as pasted input, then computes physicochemical properties, pairwise sequence similarity, and clustering visualizations to reveal how your proteins relate to one another.
Pipeline Overview
Given a set of protein sequences, the pipeline will:
- Calculate physicochemical properties — sequence length, molecular weight, amino acid composition, and isoelectric point (pI) for each protein
- Perform pairwise sequence comparisons — align every protein against every other and generate a similarity score table
- Visualize relationships — cluster proteins by physicochemical features, sequence similarity, or both, displayed as dendrograms
Try the Workflow Yourself
| Resource | Link |
|---|---|
| GitHub Repository | luquelab/Flyby-Denial |
| Google Colab Notebook | Open in Colab |